Real-time PCR for the detection and differentiation of DNA from Mycoplasma synoviae field strains and the MS-H vaccine strain in extracts from avian samples.
The kit contains all reagents and controls required for the detection of Mycoplasma synoviae field strains and vaccine strain MS-H. Amplified products are detected in real-time via a specific labelled hydrolysis probe, which is detected in a designated channel on the qPCR thermocycler. Mycoplasma synoviae field strains are detected in the FAM channel and vaccine strain MS-H is detected in the CY-5 channel. An Internal Control (IC) is detected in the HEX channel for identification of PCR inhibition to safeguard against false negative results.
In summary, the vetproof® MS-H DIVA qPCR Kit is a triplex assay enabling the simultaneous detection of:
| Species: | Poultry |
| Catalogue number: | KIT250001 |
| Classification: | Real-time PCR |
| Technique: | Real-time PCR |
| Intended use: | Detection and differentiation of Mycoplasma synoviae field strains and the MS-H vaccine strain in extracts from avian samples |
| Kit format: | 100 reactions |
| Data pack available: | Upon request |
| Standards available: | No |